Analysis of paralytic shellfish poisoning toxin congeners by a sodium channel receptor binding assay

Usup, G. and Leaw, CP and Cheah, MY and Ahmad, A. and Ng, BK (2004) Analysis of paralytic shellfish poisoning toxin congeners by a sodium channel receptor binding assay. Toxicon, 44. pp. 37-43.

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Abstract

This study was carried out to characterize the detection and quantitation of several paralytic shellfish poisoning (PSP) toxin congeners using a receptor binding assay (RBA). This involved competitive binding of the toxin congeners against tritiumlabeled STX for receptor sites on rat brain sodium channels. Competitive binding curves were described by a four-parameter logistic equation. Half-saturation values (EC50) ranged from 4.38 nM for STX to 142 nM for GTX5. Receptor binding affinity was in the order STX . GTX1/4 . neoSTX . GTX2/3 . dcSTX . GTX5, and this was similar to the order of mouse toxicity of these congeners. Predicted toxin concentrations from observed STXeq values and EC50 ratios relative to STX were within 20% or better of the actual concentrations used in the assay. In contrast predicted toxin concentrations using mouse toxicity ratios relative to STX did not provide a good match to actual concentrations, except for GTX1/4. This study has shown that the rat brain sodium channel RBA will provide a reliable integration of total toxicity of various PSP toxin congeners present in a sample.

Item Type: Article
Additional Information: Universiti Malaysia Sarawak, (UNIMAS)
Uncontrolled Keywords: Universiti Malaysia Sarawak, UNIMAS, IPTA, education, sarawak, malaysia, kuching, samarahan, universiti, univeristy
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation
Depositing User: Karen Kornalius
Date Deposited: 12 Jun 2014 07:41
Last Modified: 20 Mar 2015 03:10
URI: http://ir.unimas.my/id/eprint/3206

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