Isolation, Identification and Screening of Antimicrobial Properties of the Marine-Derived Endophytic Fungi from Marine Brown Seaweed

Changi, Wong and Proksch, Peter and Lee, Tung Tan and Samuel, Lihan and Aazani, Mujahid and Müller, M. (2015) Isolation, Identification and Screening of Antimicrobial Properties of the Marine-Derived Endophytic Fungi from Marine Brown Seaweed. Microbiology Indonesia, 9 (4). pp. 141-149. ISSN 1978-3477

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Abstract

Marine seaweeds are known to produce valuable medicinal compounds such as antioxidants and anticoagulants, and have been reported to display antimicrobial activity against gram positive and gram negative bacteria. Several studies have identified so-called endophytic fungi living inside their hosts as the source of active compounds. In this study marine brown seaweed, Padina sp., was studied with regards to their endophytic fungi to assess if they are the source of the reported antimicrobial activity. Twenty fungal isolates were isolated from Padina sp. collected off Talang-Talang Island, Sarawak, Malaysia. All isolates were screened for their antimicrobial properties and 11 out of 20 isolates displayed positive results. DNA was successfully extracted for five isolates and sequence analysis grouped all of them with other endophytic fungi. “Fungus 2” seems to be related to a so far uncultured endophytic fungus. “Fungus 19” showed the most promising antimicrobial properties and was chosen for further agar well assay and cytotoxicity testing. Its ethyl-acetate extract showed positive results in the agar well assay and also a cytotoxic effect on Artemia nauplii. The extract was screened using HPLC and showed a compound similar to a known anti-cancer compound, dihydromyricetin, which is also an anti-intoxicant, anti-inflammatory and anti-oxidative agent which may be responsible for the observed antimicrobial activity.

Item Type: Article
Uncontrolled Keywords: antimicrobial properties, endophytic fungi, Marine Brown Seaweed, Padina sp., research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 23 May 2017 04:01
Last Modified: 23 May 2017 04:01
URI: http://ir.unimas.my/id/eprint/16380

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