Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays

Cox-Singh, J. and Pomrehn, Andrea S. and Wolfe, Nathan D. and Hasan, A. Rahman and Huong-Ying, Lu and Balbir, Singh (2000) Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays. International Journal for Parasitology, 30 (11). pp. 1177-1179. ISSN 0020-7519

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Abstract

The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 microfilariae/ml) to accurately assess the limit of detection by nested-PCR. Nested-PCR consistently detected B. malayi DNA in samples with ≥10 microfilariae/ml. Post-filtration, microfilaria-depleted, blood-spots from microfilaria-positive samples were screened by nested-PCR and B. malayi specific ‘free’ DNA was detected in 51.7% of these samples. There was no evidence for ‘free’ DNA in microfilaria-negative individuals from this endemic community.

Item Type: Article
Uncontrolled Keywords: Brugia malayi; Nested-polymerase chain reaction; Sensitivity; Free DNA; Filtration, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Medicine and Health Sciences
Depositing User: Karen Kornalius
Date Deposited: 07 Apr 2017 02:08
Last Modified: 07 Apr 2017 02:08
URI: http://ir.unimas.my/id/eprint/15864

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