Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA

Wai, Chee Siong (2022) Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA. [Final Year Project Report] (Unpublished)

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Abstract

Metroxylon Sagu also known as sago plants can be found in Malaysia especially in Sarawak. The sago plants serve as starch sources for the locals. The sago plants have large economical value when the plants is trunking. But one day, scientist have found out that the trunking sago palms have diverse into another categories which are non-trunking sago palms. The non-trunking sago palms cause the economical to be reduce from time to time. The study is carry out through expression analysis to identify and differentiate the specific gene via cDNA amplification. Gene of Peroxidase A2 and Catalase was selected to design the primers. The experiment was using CTAB based method to extract the mRNA from the sago leafs. The primer selected purposely designed to apply. Expression level of the peroxidase A2 gene for trunking and non-trunking expressed equally. The expression of catalase gene cannot be expressed due to many reasons. Further research need to be done for confirmation of the related genes. CTAB based method is consume time but can get better results.

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