Wai Ling, Ooi and Son, Radu and Gulam, Rusul and Mohamed Ismail, Abdul Karim and Endang, Purwati and Samuel, Lihan (2000) Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli o157 :H7. Pakistan Journal of Biological Sciences, 3 (1). pp. 35-37. ISSN 1812-5735
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Abstract
A total of 30 strains of Escherichia coli 0157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus(ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Escherichra coli O157:H7, ERIC-PCR. |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation |
| Depositing User: | Lihan |
| Date Deposited: | 24 Aug 2022 07:37 |
| Last Modified: | 24 Aug 2022 07:37 |
| URI: | http://ir.unimas.my/id/eprint/39297 |
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