Development of serological assay for detection of Chikungunya virus infection

Ooi, Ka Beng (2018) Development of serological assay for detection of Chikungunya virus infection. [Final Year Project Report] (Unpublished)

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Abstract

Chikungunya virus (CHIKV) is a mosquito-borne virus that infects human. The infection caused by CHIKV and dengue virus (DENV) share some common signs and symptoms which includes high fever, chills, rashes, joint and muscle pain. However, the prognosis, patient care, and persistent symptoms are different. The accurate identification of CHIKV is important during CHIKV outbreak, since dengue infection is endemic in Malaysia. To date, the diagnosis of CHIKV could only be done at Institute of Medical Research (IMR) and National Public Health Laboratory (NPHL) which are located at Peninsular Malaysia. Hence, the aim of this study is to develop a serological assay for the detection of CHIKV infection by the use of a recombinant antigen. The recombinant CHIKV E2 protein are expressed in Escherichia coli (E. coli) using pET SUMO expression system. The expressed protein was evaluated by SDS-PAGE and western blot. Then the E2 recombinant protein was purified through ProBond Purification System (Novex, CA, USA) and dialysed gradually in PBS. Then, ELISA are carried out by using the E2 recombinant protein against various serum and the sensitivity and specificity are 60.7% and 90% respectively.

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