Screening, Isolation and Characterization of Plant Fungal Pathogen from Ananas comosus (Pineapple)

Muhammad Fadzil Ashraf, Abd Rahman (2018) Screening, Isolation and Characterization of Plant Fungal Pathogen from Ananas comosus (Pineapple). [Final Year Project Report] (Unpublished)

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Abstract

Ananas comosus (Pineapple fruits) have been known as constitute commercially and nutritionally important fruit in Sarawak. It is reported that 20% of fruits produced are lost to spoilage. Fungi are the major causal agent for most plant disease in which they are responsible for 70% of all major crop diseases. This research project aim to investigate on the diversity of plant fungal pathogen that always infect and affect the pineapple fruits at Kampung Sungai Mata, Samarahan, Sarawak. Four infected samples of pineapple were selected and harvested from the place. The samples were cultured on PDA plates and incubated at 28°C for 5 days. Resulting growth were screened for fungal species by using morphological and molecular identification method. Then, the isolated fungi species were undergone pathogenicity test. A total of eleven fungi species had been successfully isolated from the infected pineapples which were Curvularia clavata, Trichoderma harzianum, Fusarium oxysporum f sp. Lycopersici, Curvularia eragrostidis, Neoscytalidium dimidiatum, Lasiodiplodia theobromae, Talaromyces funiculosus, Talaromyces stollii, Fusarium fujikuroi, Geotrichum candidum, and Botryosphaeria rhodina. The highest frequency (13%) and prevalence (75%) were Fusarium oxysporum f sp. lycopersici, Neoscytalidium dimidiatum, Lasiodiplodia theobromae, and Fusarium fujikuroi. All the species were pathogenic to the Ananas comosus except Trichoderna harzianum Therefore, this indicates that ten species were responsible for the spoilage of the pineapple.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2018.
Uncontrolled Keywords: Fungi, Pathogenic, Pineapple fruit
Subjects: Q Science > Q Science (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Patrick
Date Deposited: 30 May 2021 16:36
Last Modified: 31 Jan 2024 06:37
URI: http://ir.unimas.my/id/eprint/35389

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