Molecular Characterisation of Diazotrophic and Plant Growth Promoting Rhizobacteria (PGPR) for Sago Palm (Metroxylon sagu, Rottb.)

Nurul Aainaa Shafiqah, El-Arish (2018) Molecular Characterisation of Diazotrophic and Plant Growth Promoting Rhizobacteria (PGPR) for Sago Palm (Metroxylon sagu, Rottb.). [Final Year Project Report] (Unpublished)

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Abstract

Sago palm (Metroxylon sagu, Rottb.) is one of the crops widely planted in Sarawak. The production of sago relies on the use of chemical fertilizers which contributes major concern on hazards to human health and environment. Advanced research today shows that plant growth promoting rhizobacteria (PGPR) has been characterized as diazotrophic species that shows a significant role in the sustainable agriculture development. It lives in roots of the certain plants and their surrounding soil to enhance the plant growth. Today, excessive use of chemical fertilizers slowly reduced the production of certain plant species and making PGPR as another factor to enhance the plant growth. In this study, the PGPR was isolated from roots of sago palm (Metroxylon sagu, Rottb.) and surrounding soil growing in Kampung Raeh, Serian, Sarawak. All the PGPR bacterial isolates were further screened for their PGPR traits such as nitrogen fixing activity, production of indole-3-acetic acid (IAA) and solubilization of phosphate. A total of 13 rhizobacteria was isolated and underwent in vitro screening test. Results showed 11 nitrogen fixing bacteria were isolated using Burk's nitrogen free medium and the ability to solubilize the phosphate was seen among eight isolates only using Pikovskaya agar. All isolates produced IAA in the presence of L-Tryptophan showed maximum production of26.071 µg/ml and the minimum was 0.750 µg/ml. In this study, the highest five isolates preferred Tryptophan for productions of IAA were selected for identification. The dendogram displayed relationship among the identified isolates. Molecular characterization was carried out for identification by 16S rRNA gene sequencing using universal primers of 27F and 519R respectively. The species identified was closely related to genus Marinobacter sp. (G), Roseomonas sp. (I), Enterobacter cloacae gene (J), Uncultured gammaproteobacterium clone (L) and Bacillus aryabhattai strain (M) and all showed major potential as plant growth promoting rhizobacteria for sago palm.

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