Screening of endophytic fungi isolated from medicinal plant for antagonistic properties against plant fungal pathogens and its mode of action

Carmela Eve, Johnny (2018) Screening of endophytic fungi isolated from medicinal plant for antagonistic properties against plant fungal pathogens and its mode of action. [Final Year Project Report] (Unpublished)

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Abstract

The invasion of pests and pathogens in plants has caused numerous loss in the economy and well-being of humankind. Recent studies have shown that fungi can be used as an agent in biological control. The aim of this research study is to screen the endophytic fungi against the plant fungal pathogen and to examine the volatile compounds and hydrolytic compounds. Five types of endophytic fungi which are Marasmius cladophyllus, Phlebia acanthocystis, lnonotus pachyphloeus, Penicilium pinophilum and Hypoxylon investiens and one type of plant fungal pathogen which is Phytophthora capsici were used during the entire experiment. Screening of endophytic fungi against plant fungal pathogen by dual culture assay was conducted. M. cladophyllus, P. acanthocystis, and H. investiens were the best three endophytic fungi that showed the strongest antagonistic action against the plant fungal pathogen. M. cladophyllus, P. acanthocystis, and H. investiens showed a negative result in volatile compounds test and for hydrolytic compounds test, all three endophytic fungi showed positive results for amylase activity. P. acanthocystis, and H. investiens showed positive result for lipase activity and M. cladophyllus showed negative result.

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