Detection and isolation of genes involved in degradation of heterocyclic hydrocarbon from marine bacteria

Tay, Khok Tin (2014) Detection and isolation of genes involved in degradation of heterocyclic hydrocarbon from marine bacteria. [Final Year Project Report] (Unpublished)

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Abstract

Heterocyclic hydrocarbons are among the most prevalent and harmful environmental pollutants. Aromatic compounds such as carbazole (CAR), dibenzothiophene (DBT) and biphenyl (BPH) are the pollutants that can be found in the polluted sites. Heterocyclic hydrocarbons are toxic, mutagenic and carcinogenic. Angular dioxygenase genes are the genes that responsible for the first mechanism of metabolism in aromatic compound to metabolize CAR, DBT and BPH. Paracoccus sp. M02 is previously identified bacteria that have the capability to degrade CAR, DBT and BPH. The purpose of this study is to extract the DNA of bacteria in order to detect and isolate the functional genes of bacteria involved in degradation of heterocyclic hydrocarbon. The total DNA of bacteria, Paracoccus sp. M02 was successfully extracted. Primers for functional genes detection were designed based on the genes previously reported. The presence of car genes, dbt genes and bph genes were confirmed through polymerase chain reaction (PCR) amplification. Results from PCR showed that there are bands for universal primer (27F and 1525R) and designed specific primer DbtA-F2 and DbtA-R2. The PCR product with designed specific primer DbtA-F2 and DbtA-R2 were sent for sequencing.

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