Effects of castor oil on cellulase activities during fed-batch anaerobic fermentation

Nurul Shafikah, Che Lit (2014) Effects of castor oil on cellulase activities during fed-batch anaerobic fermentation. [Final Year Project Report] (Unpublished)

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Abstract

The discovery of bioethanol production from anaerobic fennentation has been a great contributor in preventing the massive depletion of the fossil fuels. Not just that, this particular energy source can be generated from renewable carbor.ydrate sources such as wastepaper via microbial fermentation. For this study, Saccharomyces cerevisiae was used as the conversion microorganism and carboxymethylcellulose (CMC) as its substrate material. Enzyme cellulase is also crucial in this study as it convert CMC into glucose. High Performance Liquid Chromatography (HPLC) analysis is chosen as one of the analytical methods to analyse the samples collected. Based on the result obtained, the highest ethanol yield was reported at 50 FPU 2.5% CMC. As for conclusion, the castor oil observed to have effects 011 the cellulase activities- during the fennentation process and it also aids in reducing the ethanol toxicity. In order to obtain a better yield of ethanol, it was suggested that the effects· of pH towards the fermentation profile is carried out.

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