Isolation and Characterization of Heterocyclic Hydrocarbon Degrading Bacteria from Seawater

Ejal, Linting (2017) Isolation and Characterization of Heterocyclic Hydrocarbon Degrading Bacteria from Seawater. [Final Year Project Report] (Unpublished)

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Abstract

The presennce of heterocyclic hydrocarbon such as carbazole (CAR), dibenzothiophene (DBT) and dibenzofuran (DBF) in our environment leads to contamination of soil and aquatic environment. This study was conducted to isolates heterocyclic hydrocarbon degrading bacteria from seawater. The sea water sample was collected from Kampung Buntal, Kuching Sarawak. The confirmation of the presence of heterocyclic hydrocarbon degrading bacteria are conducted via enrichment techniques used ONR7a broth media as an enrichment medium that supplemented with heterocyclic hydrocarbon which is carbazole. The isolation of the bacteria degrading heterocyclic hydrocarbon was conducted using double layer ONR7a agar media supplemented with heterocylic hydrocarbons (CAR, DBT and DBF) as source of carbon and energy. Two isolated bacteria were identified from this studied which uncultured bacterium clone 070036_048 and idiomarina sp. CF5.

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