Morphological Identification of Fungi in Duck Feeds from a Duck Farm in Bako, Kuching, Sarawak

Dayang Nadila Farahana, Durahim (2017) Morphological Identification of Fungi in Duck Feeds from a Duck Farm in Bako, Kuching, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

A multiple number of dead ducks in a duck fann located in Baka, Kuching, Sarawak were found the day after the introduction of newly modified duck feeds. Plus, an apparent signs of diseases which potentially caused by toxin-producing fungi were also observed. Thus, the isolation and identification of fungi in a total of 9 suspected duck feeds were performed. The isolation of fungi in the duck feeds was made by spread-plating technique on PDA agar from the dilution of the duck feed suspensions. All of the duck feeds revealed fungal colonies count within the desired range, which then subjected to sub-culturing process, and about 24 pure fungal colonies were obtained. The identification the fungal isolates were made through the morphological examination from the macroscopic views on the PDA plates. Subsequently, the fungal isolates examined for the microscopic features by the lactophenol cotton blue (LPCB) stained wet mounting slides under compound microscope. About 9 potential genera of fungi from the fungal isolates were identified which included Alternaria, Aspergillus, Candida, Chaetomium, Fusarium, Moniliella, Mucor, Penicillium and Rhizopus. About 4 genera were expected to be potentially toxinproducing fungi which are Alternaria, Aspergillus, Fusarium and Penicillium. It was deduced that these potentially toxin-producing fungi are one of the important reasons for the potential mycotoxicoses disease in the duck farm.

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