Nuradzreen Khalida, Mohd Khalid (2017) Characterization of antimicrobial bioactive compound produced by endophytic fungi Isolated from medicinal plant, Piper betle. [Final Year Project Report] (Unpublished)
![[img]](http://ir.unimas.my/style/images/fileicons/text.png) |
PDF (Please get the password by email to repository@unimas.my , or call ext: 082-583914/3973/3933)
Khalida.pdf Restricted to Registered users only Download (86MB) |
Abstract
In recent years, the world health problems are caused by the overuse of antibiotics, resulting the development of resistance towards drugs against pathogenic microorganisms. Hence, this research was designed to characterize the antimicrobial bioactive compound produced by endophytic fungi isolated from Piper betle. P. betle acts as a therapeutic agent to treat various diseases, where it contains abundant of bioactive compounds. The endophytes isolated from P. betle were analysed against four test microorganisms. The morphological and molecular characterization employing a Polymerase Chain Reaction (PCR) were conducted to establish their identities. These fungi were mass cultivated for II days and the compounds produced were harvested. Subsequently, invitro antimicrobial screening was conducted to screen for the antimicrobial activity. Followed by disc diffusion method, where the zone of inhibition was determined. An antimicrobial activity of ethyl acetate extract was observed except for Escherichia coli. However, no antimicrobial activity in methanolic extract was observed. Hence, the stock solution were used, resulted in E. coli and Pseudomonas aeruginosa that showed inhibition. The Minimal Inhibition Concentration (MlC) values were further assessed by using broth microdilution method. The readings obtained by ELISA reader showed an antimicrobial activity in extracts of ethyl acetate (10 mg/ml) and methanol (50 mg/ml). This indicates that a variation in susceptibility level of microorganisms towards the fungal extracts. Hence, the antimicrobial bioactive compound produced by endophytic fungi isolated from Piper betle have a potential to be used as antimicrobial agents to fight against pathogenic microorganisms.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2017. |
| Uncontrolled Keywords: | Piper betle, pathogenic, antimicrobial activity, PCR, MIC, ELISA reader, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Patrick |
| Date Deposited: | 30 Jun 2020 10:13 |
| Last Modified: | 07 Feb 2024 06:48 |
| URI: | http://ir.unimas.my/id/eprint/30130 |
Actions (For repository members only: login required)
 |
View Item |
