Callus induction of aquilaria microcarpa baill. using leaf and nodal explants

Nur-Amalina, Binti Johari. (2016) Callus induction of aquilaria microcarpa baill. using leaf and nodal explants. [Final Year Project Report] (Unpublished)

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Abstract

Aquilaria microcarpa is one of the important tropical forest trees from Thymelaeaceae family which are the principal source of agarwood. Agarwood are expensive resinous heartwood that widely used for incense, perfume, and medicine. The increase of agarwood demand from year to year leads to uncontrolled illegal harvest of this plant from its natural habitat. Essential oil of agarwood can be extracted from the callus through suspension culture. In this study, leaf and node explants were placed into solid MS media supplemented with different concentrations of 2,4-D (2, 4- dichlorophenoxyacetic acid) alone at 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg/L and also 2,4-D plus 0.5 mg/L BAP (6- benzylaminopurine) for callus induction. Within ten weeks of culture, both explants were successfully induced callus in solid MS media supplemented with 2.0 mg/L 2,4-D alone and 2.0 mg/L 2,4-D plus 0.5 mg/L BAP. Fully developed callus were sub-cultured into fresh MS medium every four weeks to promote callus growth. The calli formed were transferred into liquid MS media with 2.0 mg/L 2,4-D plus 0.5 mg/L BAP for suspension culture. Four concentrations of sucrose 20, 30, 40 and 50 g/L were tested. Based on the observation, liquid MS media with 40 g/L sucrose showed the best callus growth.

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