Comparative expression analysis of the ribosomal protein gene, RPS15 between normal and tumor cell lines of nasopharyngeal carcinoma

Chee, Cassandra Sheau Mei (2015) Comparative expression analysis of the ribosomal protein gene, RPS15 between normal and tumor cell lines of nasopharyngeal carcinoma. [Final Year Project Report] (Unpublished)

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Abstract

Cancer is associated in a complex alteration in gene expression patterns in the ribosomal proteins that include many different ribosomal genes. Ribosomal proteins have extraribosomal functions and can be involved in other variety of activities other than protein synthesis. The differences in the gene expression of an individual ribosomal protein can affect a specific cell and cause a transformation in the cell, which could result in cell death or proliferation. The RPS15 gene is up regulated in poorly differentiated nasopharyngeal carcinoma (NPC). Our research aim is to detect different expression patterns in NPC cell lines and normal human nasopharyngeal cell line with the incorporation of Reverse Transcriptase PCR (RT-PCR). This study reveals specific PCR products with the estimated size of 115bp for RPS15. The RPS15 gene in NPC cell line was found to be up regulated in comparison to normal human nasopharyngeal cell lines

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