Production of crude cellulase from Aspergillus flavus under Solid State Fermentation (SSF) using different agro waste as substrates

Siti Najibah, Zukefli (2015) Production of crude cellulase from Aspergillus flavus under Solid State Fermentation (SSF) using different agro waste as substrates. [Final Year Project Report] (Unpublished)

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Abstract

Cellulases are a group of hydrolytic enzymes that have high commercial values in various industries such as textile, food, beverages, paper and pulp industries. Solid state fermentation method was used in this study as it is simple, more economical and productive compared to the submerged fermentation. In this study, the cellulase production produced by Aspergillusflavus was studied under solid state fennentation using oil palm empty fruit bunch (OPEFB), pineapple leaves and sago hampas as the substrates. The content of lignocellulosic materials mainly cellulose in these agricultural wastes act as the inducer of cellulase production. Solid state fermentation parameters such as incubation time (4 days -10 days), initial moisture content (65% -80%) and temperature (25°C -40°C) were investigated. From this research, it is found that the most optimum SSF conditions to produce cellulase are different among three substrates used. The present study also proved the feasibility of sago hampas as the best substrate for cellulase production among other substrates used.

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