Transformation of Chitinase in Yeast Pichia Pastoris GS115

Rasyidah, binti Ramlee (2015) Transformation of Chitinase in Yeast Pichia Pastoris GS115. [Final Year Project Report]

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Abstract

Chitinases are enzyme that degrades chitin, the second most abundant polysaccharide after cellulose. Chitin is found in the exoskeleton of many organisms including insects, fungi, yeast and algae. It is also has been found in the internal structures of other vertebrates. Chitin and the chitinolytic enzymes are very useful for their applications in biotechnology sector. Chitinase contribute in human health care especially in human disease like asthma. Chitinases also have a wide-range application in preparation of pharmaceutically important chitooligosaccharides and N-acetylglucosamin. Transformation of chitinase gene in yeast Pichia pastoris GS115 was performed via EasyCompTM transformation. Chitinase gene extracted from Metroxylan sagu and has been constructed in pPICZαC was used in this project. The confirmation of insertion in the positive transformant that contain pPICZαC/Chitinase vector was done via colony PCR.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Chitinase, chitin, pPICZαC, GS115, Metroxylan sagu, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 29 Jan 2016 02:02
Last Modified: 13 Mar 2024 05:49
URI: http://ir.unimas.my/id/eprint/10426

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