Zulkifli, Y. and Alitheen, N.B. and Son, Radu and Raha, A.R and Samuel, Lihan and Yeap, S.K and Nishibuchi, M. (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16. pp. 141-150. ISSN 1985-4668 (Print), 2231-7546 (Online)
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Abstract
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | V. parahaemolyticus, seafood, RAPD-PCR, ERIC-PCR, genomic fingerprinting, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 07 Jan 2016 07:31 |
| Last Modified: | 26 Jan 2023 04:55 |
| URI: | http://ir.unimas.my/id/eprint/10108 |
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