Evaluation and optimization of DNA extraction method from plant species leaf for polymerase chain reaction analysis

Zulaikha, binti Zainal (2012) Evaluation and optimization of DNA extraction method from plant species leaf for polymerase chain reaction analysis. [Project Report] (Unpublished)

[img]
Preview
PDF
Evaluation and Optimization of DNA Extraction Method from Plant Species Leaf for Polymerase Chain Reaction Analysis (24pgs).pdf

Download (8MB) | Preview
[img] PDF
Evaluation and Optimization of DNA Extraction Method from Plant Species Leaf for Polymerase Chain Reaction Analysis (full).pdf
Restricted to Registered users only

Download (16MB)

Abstract

DNA extraction in plant genetic studies is essential components for understanding molecular study and biology applications. Genomic DNA was extracted from 5 different selected plant species which are Capsicum annuum L.. Curcuma longa L .• lasminum officinale L.. Carica papaya L., and PsophocalplIS tetragonolobus L. by using two DNA extraction protocols which are CTAB extraction method and protocols without using liquid nitrogen. Leaves were fixed in different fixing solutions containing absolute alcohol (99.9%), chloroform and EDT A, without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen which used in breaking the cell wall. As a result, the DNA concentration in the A260/280 ratio with liquid nitrogen was in range l.9-2.15 and with fixing solutions was 1.1-l.9. Good quality DNA from fresh leaf was obtained without using liquid nitrogen and was successfully amplified by polymerase chain reaction using OPDF-8 primer. The same protocols also can be useful for extracting high molecular weight DNA from other plant materials containing large amounts of secondary metabolites and essential oils.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Polymerase chain reaction, DNA polymerases, DNA isolation, CTAS, Genomic DNA, PCR, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 01 Jul 2015 04:16
Last Modified: 01 Jul 2015 04:16
URI: http://ir.unimas.my/id/eprint/8152

Actions (For repository members only: login required)

View Item View Item