Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system

Chia, Christine Pek Imm (2007) Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system. [Project Report] (Unpublished)

[img]
Preview
PDF
Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system (24pgs).pdf

Download (328kB) | Preview
[img] PDF
Isolation and Restriction Endonuclease (RE) Site Characterization of Human 20-KDA Myosin Light Chain 2 (MLC-2) Gene From Human Colorectal System (full text).pdf
Restricted to Registered users only

Download (1MB)

Abstract

Colorectal cancer progresses in a stepwise manner and is found to be linked with the accumulating mutations (Franks and Teich, 1993). The recent discovery found that the expression of human 20-kDa Myosin Light Chain (MLC-2) gene is downregulated in the tumour colon tissue compared to the normal colon tissue (Sim et al., 2006). The objective of this study is to screen for mutation in the MLC-2 gene of the normal and tumour samples by restriction endonucleases. Total RNA of both samples was used as the starting material and was transcribed into cDNA using M-MLV reverse transcriptase. The 251 bp MLC-2 gene was successfully amplified by polymerase chain reaction and was subsequently digested with 15 different restriction endonucleases. The digestion yielded no abnormal cleavage of the MLC-2 gene fragment between tumour and normal colon samples.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) - Universiti Malaysia Sarawak, 2007.
Uncontrolled Keywords: Restriction enzymes, DNA,Unimas, university,universiti, Borneo, Malaysia, Sarawak,Kuching, Samarahan,ipta,education, undergraduate,research ,Universiti Malaysia Sarawak
Subjects: R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 25 May 2015 04:22
Last Modified: 25 May 2015 04:22
URI: http://ir.unimas.my/id/eprint/7867

Actions (For repository members only: login required)

View Item View Item