Establishment of axenic culture and induction of organogenesis in kapur bukit (dryobalanops beccarii dyer)

Mastura, Sani (2010) Establishment of axenic culture and induction of organogenesis in kapur bukit (dryobalanops beccarii dyer). [Project Report] (Unpublished)

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Abstract

Dryobalanops beccarii Dyer (Kapur Bukit) has been recommended by Sarawak Forest Department as one of the four timber species for forest plantation in Sarawak (Thai, 2002) and clonal materials are preferred for this purpose. However, the production of large quantity of planting stock through conventional propagation has not been very successful as Kapur Bukit posses recalcitrant seed and is irregular in flowering and thus fruiting. The in vitro technique therefore has been considered as an alternative for mass production of clonal material. Nevertheless, previous studies had shown that, it was difficult to establish aseptic and viable explants in culture because of browning and contamination. In 2009, Ang has found that phenolic released from petiolule explants were negligible. Roots also were successfully induced from friable callus that derived from this explants. To improve the percentage of axenic and viable explants in culture, further research was done by modifying the disinfection protocol that previously developed. Organogenesis of Kapur Bukit also was carried out by using various types and concentration of cytokinins, either alone or in combination with auxin to induce shoot formation. From this study, it was found that organogenesis of root was successfully induced at concentration 0.5, 1.0, 1.5, 2.0 mg/L of 2, 4-D. While green callus was observed in 4 mg/L BAP, 2 mg/L BAP+ 1 mg/L Kin and 3 BAP+ 0.5 mg/L Kin supplemented WPM medium. However, no sign of shoot formation was observed as phenolic that released caused green callus died after 3 months cultured. Seems browning problem is more critical than microbial contamination.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) - Universiti Malaysia Sarawak, 2010.
Uncontrolled Keywords: Plant embryology , Plant morphogenesis, browning, organogenesis, petiolule explants, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, Universiti Malaysia Sarawak
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 22 Jun 2015 03:44
Last Modified: 22 Jun 2015 03:44
URI: http://ir.unimas.my/id/eprint/7840

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