Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products

Fasihuddin Badruddin, Ahmad and Mohammed, Akkbik and Zaini Bin, Assim (2011) Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products. International journal of analytical chemistry, 2011. pp. 1-10. ISSN 1687-8760 (Print), 1687-8779 (Online)

[img]
Preview
PDF
Optimization and Validation of RP-HPLC-UVVis Method for Determination Phenolic Compounds in Several Personal Care Products.pdf

Download (1MB) | Preview

Abstract

An HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methyl cinnamate (OMC) as UVB-filter in several personal care products. The dynamic range was between 1 to 250 mg/L with relative standard deviation less than 0.25% (n = 4). Limits of detection for BHA, BHT, and OMC were 0.196, 0.170, and 0.478 mg/L, respectively. While limits of quantification for BHA, BHT, and OMC were 0.593, 0.515, and 1.448 mg/L, respectively. The recovery for BHA, BHT, and OMC was ranged from 92.1–105.9%, 83.2–108.9%, and 87.3–103.7%, respectively. The concentration ranges of BHA, BHT, and OMC in 12 commercial personal care samples were 0.13–4.85, 0.16–2.30, and 0.12–65.5mg/g, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation, that is, 0.0004–10 mg/g, 0.002–5mg/g, and up to 100 mg/g for BHA, BHT, and OMC, respectively.

Item Type: Article
Uncontrolled Keywords: Optimization, Validation, RP-HPLC-UV, FSTS, 2011, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, research
Subjects: Q Science > QD Chemistry
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 05 May 2015 03:05
Last Modified: 25 May 2015 07:04
URI: http://ir.unimas.my/id/eprint/7329

Actions (For repository members only: login required)

View Item View Item