Lactic Acid production using alkaliphile lactic acid bacterium

Amirul, Bin Mahmud (2012) Lactic Acid production using alkaliphile lactic acid bacterium. [Project Report] (Unpublished)

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Abstract

Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and presence of contamination were investigated. Batch fermentation system was done at 37 °C and agitated at 250 rpm with controlled pH environment. Fermentation at pH 9 gave high production biomass and lactic acid of about 1.80 g/L and 45.21 g/L while less production is shown at pH 10 with 1.27 g/L and 42.66 g/L. Spread plate of both fermentation show growth only single colony which no contamination. Consequently, it shows that high pH affect cell growth, lactic acid production and prevents contamination. Complete fermentation at pH 9 show is more favourable with high rate production of lactic acid in 42 h compared to at pH 10 in 72 h.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Lactic acid bacteria, high pH, batch fermentation, lactic acid, alkaliphile lactic acid bacteria, glucose, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 15 Jan 2015 03:08
Last Modified: 22 Sep 2015 02:43
URI: http://ir.unimas.my/id/eprint/6206

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