Isolation and characterization of carbazole-degrading bacteria from mangrove environment

Kho, Tze Chiat (2012) Isolation and characterization of carbazole-degrading bacteria from mangrove environment. [Project Report] (Unpublished)

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Abstract

Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to toxic activities that will lend serious environmental problems. This study was conducted to isolate and characterize carbazole-degrading bacteria from mangrove environment. The samples were collected from Bako National Park and the presence of carbazole-degrading bacteria was confirmed through enrichment culture of the samples. Screening of the bacteria was successfully done by observing the growth of the strains on ONR7a agar containing carbazole as sole carbon source. Four different strains of carbazole degrading bacteria that are able to grow on carbazole source were isolated. Isolate strains were characterized using phenotypic and molecular techniques. From the BLAST search, four different strains of carbazole degrading bacteria were identified as Sphingomonas sp., Flavobacterium sp., Gaetbulibacter sp., and Thalassospira sp. with maximum identity of 100%, 97%, 99%, and 99% respectively. All strains appeared as a non-motile Gram-negative rod shaped bacterium

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Heterocyclic compounds--Synthesis, Organic cyclic compounds--Synthesis, Macrocyclic compounds--Synthesis, Carbazole, Carbazole-degrading bacteria, 16S rRNA, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research.
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 15 Jan 2015 02:07
Last Modified: 23 Sep 2015 02:09
URI: http://ir.unimas.my/id/eprint/6198

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