DNA genotyping of Duabanga moluccana (Sawih) using ISSR Marker

Cinderella, Anak Sio (2012) DNA genotyping of Duabanga moluccana (Sawih) using ISSR Marker. [Project Report] (Unpublished)

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Abstract

The genetic diversity of Duabanga moluccana in 3 populations was characterized using inter-simple sequence repeats (ISSR) technique. Two ISSR primers: CAG(CA)8 and (ACC)6G were screened, of which both are polymorphic and provide informative patterns to determine genetic relationships. ISSR amplification was conducted on 90 samples from 3 populations, and all 40 loci detected were polymorphic. The percentage of PPL at Mukah, Niah and Tatau were 82.5%, 90.00% and 62.50% respectively, with an average of 78.33%. Nei’s gene diversity (h) and Shannon’s Information Index (I) of D. moluccana at the species level were 0.2206 and 0.3569, respectively. The genetic differentiation coefficient (Gst) among populations was 0.1234. The gene flow (Nm) among populations was 1.7759, indicating that gene flow was high among populations of D. moluccana.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Gene expression, Genetic regulation, D. moluccana, ISSR, Nei’s gene diversity, Shannon’s Information Index, polymorphic, gene flow, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research.
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 14 Jan 2015 06:38
Last Modified: 22 Sep 2015 04:24
URI: http://ir.unimas.my/id/eprint/6174

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