Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm

Famila Farvin, Binti Ghulam Dasthagir (2012) Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm. [Project Report] (Unpublished)

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Abstract

Enterobacteriaceae is a member of gram-negative bacilli and contain more than 30 genera. They act as the main indicator for the bacteriological water quality. Study was conducted to determine the occurrence of Enterobacteriaceae family in Empurau (Tor Tambraides) pond at Indigenous Fish Research & Production Centre (IFRPC) in Tarat, Sarawak in which this it could reflect the level of microbe found in the pond. Studies also been conducted for detection on any possible pathogenic bacteria that might cause infectious disease in fish pond. Besides that, studies were conducted for determining the relationship between physicochemical parameter and microbiological parameter with the development of the fish in the pond. Water sample from the pond were collected and analyzed for the presence of Enterobacteriaceae. Sample plated on Nutrient Agar and colonies formed were tested on gram staining. Colonies with Gram-negative were further analyzed with (GTG)5 PCR. The phylogenetic tree was constructed based on the DNA fingerprinting to illustrate the relationship among the bacteria isolates. 11 randomly picked samples were tested with API 20E kit for identification of the bacteria.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Enterobacteriaceae, Enterobacteriaceae--Identification, identification, microbiological activities, empurau fish, (Tor Tambraides), (GTG)5 PCR analysis, API 20E kit, FSTS, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: Q Science > QE Geology
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 06 Jan 2015 02:08
Last Modified: 23 Sep 2015 00:41
URI: http://ir.unimas.my/id/eprint/6045

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