Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development

Chuo, Winnie (2011) Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development. [Project Report]

[img]
Preview
PDF
Molecular Cloning and Expression Study of Proline Rich ENAVASP Ligand 2A (PREL-2A) Gene in Zebrafish Development (24pgs).pdf

Download (208kB) | Preview

Abstract

Proline rich Ena/VASP Ligand 2 (PREL 2) is designated as Lamellipodin (Lpd) in mammals. PREL-2 possesses the ability to colocalize with Ena/VASP proteins at the tips of lamellipodia. Mammalian PREL-2 was found to have significant functions on cell signalling, direct migration, promoting axon guidance, affecting adhesion and regulating lamellipodial dynamics. Zebrafish PREL-2 gene is duplicated into two copies namely, PREL-2a and PREL-2b during a genome duplication event. The expression data of PREL-2 family only available for selected adult organs in human and mice but still no detailed information of PREL-2a in the embryonic stages. In this study, the cloning and expression profile of zebrafish PREL-2a gene was described. Semiquantitative RT-PCR analysis revealed that zebrafish PREL-2a gene is expressed throughout the development. PREL-2a gene expression was first expressed at 1K cells stage (3hpf) and constantly expressed throughout zebrafish development up to 5dpf. The RT-PCR result indicated that PREL-2a gene show maternal and zygotic expression.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Zebrafish, PREL-2a gene, RT-PCR, gene expression, Zebra danio--Development, carp, Cyprinidae, FSTS, 2011, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 03 Nov 2014 06:19
Last Modified: 25 May 2015 06:24
URI: http://ir.unimas.my/id/eprint/5333

Actions (For repository members only: login required)

View Item View Item