A preliminary phylogenetic analyses of schismatoglottis calyptrata complex utilizing nuclear ITS region

Nurul Fatini Shahirah, binti Suharizan (2012) A preliminary phylogenetic analyses of schismatoglottis calyptrata complex utilizing nuclear ITS region. [Project Report]

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Abstract

Sehismatoglottis ealyptrata complex (Schismatoglottideae: Araceae) consist of at least 40 species. This study focused on six accessions of Sehismatoglottis calyptrata complex from Kuching, Sibu and Selangor. Molecular investigation was carried out to resolve the relationship between the species investigated. The nuclear ITS region located in nuclear ribosomal DNA (nrDNA) were used for this study. DNA was extracted from dried leaf using CTAB method. Extracted DNA were amplified and purified before sending for sequencing at a commercial company. The sequences obtained were checked manually and aligned in BioEdit ver 7.0.5 together with an outgroup, Phi/onotion americanum. Phylogeny analyses were carried out in PAUP ver v.4.0b10 for maximum parsimony and RAxML for maximum likelihood. Maximum parsimony and maximum likelihood analyses showed strong and moderate bootstrap support (MP: 100%, ML: 43%) for the ingroups excluding Schismatoglottis calyptrata AR 3586. This study shows that all the species sampled from Sarawak are closely related based on the molecular evidence.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Araceae, Schismatoglottis calyptrata complex, molecular investigation, phylogenetic, ITS region, Araceae, 2012, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: Q Science > QK Botany
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 20 Oct 2014 03:08
Last Modified: 12 May 2015 01:51
URI: http://ir.unimas.my/id/eprint/5066

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