Species Identification of Wildlife Meats Using Mitochondrial Cytochrome Mitochondrial Cytochrome B Gene

Licia, Ho and Abdullah, M.T and Esa, Y. (2014) Species Identification of Wildlife Meats Using Mitochondrial Cytochrome Mitochondrial Cytochrome B Gene. UNSPECIFIED. Universiti Malaysia Sarawak, (UNIMAS). (Submitted)

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Abstract

Species identification by using mitochondrial DNA is a convenient methodfor species identification of wildlife meat samples. In this study,mitochondrial DNA (mtDNA) partial cytochrome b gene was used toidentify the species origin of 25 putative meat samples provided by theSarawak Forestry Corporation (SFC). All samples were suspected to beillegal removed from conservation animals under the Wild Life Protection Ordinance 1998. DNA was extracted by using modified cetyltrimethylammonium bromide (CTAB) protocol. DNA sequences obtained were compared with DNA sequence of reference samples in GenBank by performing BLAST search. Approximately 14 out of 25putative samples were successfully extracted and analyzed. The results showed that all 14 putative samples displayed more than 90% similarity with the reference samples in GenBank. Among the 14 putative samples, nine samples were from soft-shelled turtle, two samples were from wild boar, and each of one sample were from barking deer, civet, and flying fox respectively. All the 14 samples identified belong to the protected species category.

Item Type: Monograph (UNSPECIFIED)
Additional Information: Universiti Malaysia Sarawak, UNIMAS
Uncontrolled Keywords: Species identification, putative meat, cytochrome b. gene, wildlife forensic, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: A General Works > AC Collections. Series. Collected works
A General Works > AC Collections. Series. Collected works
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 08 May 2014 02:30
Last Modified: 10 Sep 2015 06:53
URI: http://ir.unimas.my/id/eprint/2364

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