Isolation of genomic dna from fruit bats of kelabit Highlands for dna archiving and determination of Genetic variation

Mohd Azib, Salleh and Edmund Sim, Ui Hang and Mustaffa, Abdul Rahman and Mohd Tajuddin, Abdullah (1999) Isolation of genomic dna from fruit bats of kelabit Highlands for dna archiving and determination of Genetic variation. In: UNSPECIFIED. (Unpublished)

[img]
Preview
PDF
Isolation of genomic dna from fruit bats of kelabit Highlands for dna archiving and determination of Genetic variation.pdf

Download (12kB) | Preview
Official URL: http://www.eldis.org/

Abstract

A total of 40 fruit bats belonging to five species, namely Cynopterus brachyotis (34), Aethalops alecto (3), Pipistallus tenius (1), Balionycteris maculata (1) and Megaerop ecaudatus (1) were captured from two sites during the expedition to Bario in April 1995. Whole blood and liver tissue samples were taken from freshly killed bats and stored in liquid nitrogen in the field before transferring to a - 70ºC deep freezer in the laboratory. Genomic DNA was successfully isolated from whole blood samples derived from 13 bats using SDS-lysis followed by phenol-extraction methods. Pure DNA samples were dissolved in tris-EDTA buffer and stored at -20ºC for DNA archiving and determination of genetic variations within and between the different species

Item Type: Conference or Workshop Item (UNSPECIFIED)
Additional Information: Universiti Malaysia Sarawak, UNIMAS
Uncontrolled Keywords: Isolation, genomic dna, fruit bats, kelabit Highlands, DNA archiving, genetic variation, 1999, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: A General Works > AC Collections. Series. Collected works
A General Works > AC Collections. Series. Collected works
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 06 May 2014 03:17
Last Modified: 05 Oct 2015 02:53
URI: http://ir.unimas.my/id/eprint/2286

Actions (For repository members only: login required)

View Item View Item