Genetic variation in several populations of puntius schwanenfeldii using isozymes analysis, pcr-relp analysis and dna sequence analysis of cytochrome B mtdna gene

Kamarul Rahim, bin Kamarudin. (2003) Genetic variation in several populations of puntius schwanenfeldii using isozymes analysis, pcr-relp analysis and dna sequence analysis of cytochrome B mtdna gene. [Final Year Project Report] (Unpublished)

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Abstract

Genetic variation was examined between three different samples of PUnlius schwanenfeldii (Bleeker): lampam sungai fish from Serting Hilir, Negeri Sembi Ian and both local type tengadak fish and Indonesian type tengadak fish from Kapit, Sarawak by using isozymes analysis, PCR-RFLP analysis and DNA sequence analysis of cytochrome b mtDNA gene. The results obtained from these analyses were compared with each other. Lampam sungai fish from Serting Hilir, Negeri Sembilan and both local type tengadak fish and Indonesian type teugadak fish from Kapit, Sarawak are identical in morphology. The results from phylogenetic analysis showed the existence of high genetic variation between local type tengadak fish and Indonesian type tengadak fish from Kapit, Sarawak but this fmding still could not support the assumption that local type tengadak fish and Indonesian type tengadak fish were suspected to be separate species, due to small number of samples involved. Furthermore, this study also showed that lampam sungai fish from Serting Hilir, Negeri Sembilan were totally separate from both local type tengadak fish and Indonesian type tengadak fish from Kapil, Sarawak due to apparent genetic variation between the se two populations. Ho\vever, further studies with higher amount of samples from other populations need to be done to verify the results from this stud y. Isozymes analysis revealed unreliable results due to laboratory protocol problems while PCR-RFLP analysis showed no polymorphism between samples of lampam sungai fish from Serting Hilir and both Jocal type tengadak fish and Indonesian type lengadak fish from Kapil, Sarawak. In general, Ihis study indicated thaI DNA sequence analysis has pOlential advantages over isozymes analysis and PCR·RFLP analysis of cytochrome b mtDNA gene in detection of even small genetic variation, or synonymous substitution between ssmples.

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