Husna, Hj. Mohd Noor. (2003) Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002. [Final Year Project Report] (Unpublished)
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Abstract
This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylase enzyme was undertaken by microbial degradation of the media that produced clear haloes around the colonies indicated amylolytic and cellulolytic activity. These colonies were selected for molecular analysis; genomic DNA extraction, restriction endonuclease digestion, Polymerase Chain Reaction (PCR), and genomic cloning. Since there are some problems, a gene encoding extracellular a-amylase enzyme could not be cloned and sequenced.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project report (B.Sc.) - Universiti Malaysia Sarawak , 2003 |
| Uncontrolled Keywords: | Bacillus amyloliquefaciens, amylolytic, cellulolytic, a-amylase, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Saman |
| Date Deposited: | 04 Oct 2017 03:34 |
| Last Modified: | 27 Nov 2023 09:04 |
| URI: | http://ir.unimas.my/id/eprint/17930 |
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