Quantitative analysis of the expression of p53 gene in colorectal carcinoma by using real-time PCR

Bong, Ivyna Pau Ni and Lim, Patricia and Balraj, Pauline and Sim, Edmund U. H. and Zubaidah, Zakaria (2006) Quantitative analysis of the expression of p53 gene in colorectal carcinoma by using real-time PCR. Tropical Biomedicine, 23 (1). pp. 53-59. ISSN 0127-5720

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Abstract

Colorectal carcinoma ranks third among ten leading causes of cancer in Malaysia. The colorectal carcinoma tumourigenesis involves the inactivation of tumour suppressor genes, and activation of proto-oncogenes. The p53 is one of the tumour suppressor genes that is involved in the colorectal carcinogenesis. The p53 gene is located on human chromosome 17p13.1 and comprises of 11 exons. Deficiencies in the p53 gene can cause the cancerous cells to spread to distant organs such as liver, lungs, lymph nodes, spine and bone. The most common p53 abnormalities that can lead to the metastasis of colorectal tumours are mutation and deregulation of the gene. In this study, nine colorectal carcinoma samples were used to establish a simple and sensitive strategy in the study on in vivo p53 expression by using realtime LightCycler SYBR Green I technology.

Item Type: Article
Uncontrolled Keywords: colorectal carcinoma, cancer, tumor, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 18 Jul 2017 07:04
Last Modified: 18 Jul 2017 07:04
URI: http://ir.unimas.my/id/eprint/16917

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