An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf>

Foo, K.L. and Muhammad Fayyaz, Kashif and Tan, S.J. and Hashim, U. (2017) An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf>. Microsystem Technologies, 23 (5). pp. 1237-1245. ISSN 09467076

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Abstract

Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 ± 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (–SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using µAUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization. © 2016, Springer-Verlag Berlin Heidelberg.

Item Type: Article
Uncontrolled Keywords: electrochemical DNA biosensor, gold‑thiolate, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions: Academic Faculties, Institutes and Centres > Faculty of Engineering
Depositing User: Ibrahim
Date Deposited: 20 Jun 2017 00:40
Last Modified: 20 Jun 2017 00:40
URI: http://ir.unimas.my/id/eprint/16688

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