Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia

Inke, ND Lubis and Hendri, Wijaya and Munar, Lubis and Chairuddin P, Lubis and Paul Cliff, Simon Divis and Khalid, B Beshir and Sutherland, Colin J (2017) Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia. The Journal of Infectious Diseases. ISSN 1537-6613

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Official URL: https://www.researchgate.net/publication/313777791

Abstract

Background As Indonesia works towards the goal of malaria elimination, information is lacking on malaria epidemiology from some western provinces. As a basis for studies of antimalarial efficacy, we set out to survey parasite carriage in three communities in North Sumatera Province. Methods A combination of active and passive detection of infection was carried out among communities in Batubara, Langkat and South Nias regencies. Finger-prick blood samples from consenting individuals of all ages provided blood films for microscopic examination and blood spots on filter paper. Plasmodium species were identified by nested PCR of rRNA genes, and a novel assay which amplifies a conserved sequence specific for the sicavar gene family of P. knowlesi. Results 614 of 3,731 participants (16.5%) were positive for malaria parasites by microscopy. PCR detected parasite DNA in samples from 1,169 individuals (31.3%). In total, 377 participants (11.8%) harboured P. knowlesi. Also present were P. vivax (14.3%), P. falciparum (10.5%) and P. malariae (3.4%). Conclusions Amplification of sicavar is a specific and sensitive test for the presence of P. knowlesi DNA in humans. Subpatent and asymptomatic multi-species parasitaemia is relatively common in North Sumatera, and so PCR-based surveillance is required to support control and elimination activities.

Item Type: Article
Uncontrolled Keywords: Malaria; Indonesia; Plasmodium knowlesi, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Medicine and Health Sciences
Faculties, Institutes, Centres > Faculty of Medicine and Health Sciences
Depositing User: Karen Kornalius
Date Deposited: 15 May 2017 04:22
Last Modified: 25 May 2017 00:41
URI: http://ir.unimas.my/id/eprint/16221

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