Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)

Katrina, binti Aslan Joe (2012) Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007). Masters thesis, Universiti Malaysia Sarawak, (UNIMAS).

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Abstract

Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated between 1997 and 2008 were successfully sequenced. There were 25 sets of full CVA16 genome primers which were designed in such a way that it targets the whole genome of CVA16 regions in addition to three anti-sense gene specific primers that were used to amplify the 5'UTR region and two sense gene-specific primers that were used to amplify the 3'UTR region of the CVA16 genome. These primers successfully amplified the expected product size from extracted RNA of all 22 isolates.

Item Type: Thesis (Masters)
Additional Information: Thesis (M.Sc.) -- Universiti Malaysia Sarawak, 2012.
Uncontrolled Keywords: Children, Diseases, Case studies, Pediatrics, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak
Subjects: R Medicine > RJ Pediatrics
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Saman
Date Deposited: 07 Apr 2017 02:38
Last Modified: 07 Apr 2017 02:39
URI: http://ir.unimas.my/id/eprint/15886

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