Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Cinnamic: Extraction of Cinnamic Acid from Spiked Blood Plasma

Leong, Alvin Joke Chow and Showkat, Ahmad Bhawani (2016) Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Cinnamic: Extraction of Cinnamic Acid from Spiked Blood Plasma. International Journal of Polymer Science, 2016. ISSN 16879422

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Abstract

The molecular imprinting technique is used to create the molecularly imprinted polymers (MIPs) with higher binding capacity towards the template. In this research precipitation polymerization method with non-covalent approach was used to synthesize imprinted polymer microspheres. The polymerization reaction was conducted in a flask containing acetonitrile as a porogen, cinnamic acid as a template (T), acrylic acid (AA) as a monomer, divinylbenzene (DVB) as a cross-linker and azobisisobutyronitrile as an initiator. The polymer particles were characterized by using SEM and FTIR. The rebinding efficiency was conducted by batch binding assay and the results were monitored by using HPLC. The batch binding results suggested MIP1 (T: AA: DVB, 1: 6: 20 molar ratio) is most suitable compostion for the rebinding of cinnamic acid. The highly selective polymer (MIP1) was used for the extraction of cinnamic acid from human plasma. The extraction efficiency of imprinted polymer of cinnamic acid from spiked plasma was above 75%.

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