Lim, H.C. and Teng, S.T. and Lim, P.T. and Wolf, M. and Leaw, CP (2016) 18S rDNA phylogeny of Pseudo-nitzschia (Bacillariophyceae) inferred from sequence-structure information. Phycologia, 55 (2). pp. 134-146. ISSN 00318884Full text not available from this repository.
We explored the 18S rDNA sequences of Pseudo-nitzschia and its close relatives, together with their individual secondary structure information to reconstruct phylogenies. Forty-eight taxa classified in the Bacillariaceae and Achnanthaceae were analyzed by character-based (maximum parsimony and maximum likelihood) and distance-based (neighbour-joining) methods. Three raphid diatoms from the Eunotiaceae were used as out-groups. The sequencestructure alignment was generated by 4SALE (i.e. a one-letter encoded alignment that contained the individual secondary structure information) and applied to the character-based phylogeny reconstruction. All analyses highly support Pseudonitzschia + Fragilariopsis as monophyletic. The Pseudo-nitzschia/Fragilariopsis clade is further divided into three subclades. One clade comprises members in the P. seriata and P. americana complexes and species of Fragilariopsis; the second and third clades constitute members in P. pseudodelicatissima and P. delicatissima complexes. Among all analyses, the distance-based method yielded a more highly resolved phylogenetic framework for the Pseudo-nitzschia/Fragilariopsis clade than did the character-based analyses. Other than those currently known genetic markers, 18S rDNA was also useful in exploring the intrageneric relationships.
|Uncontrolled Keywords:||18S rDNA; 4SALE; Alignment; Pseudo-nitzschia; Secondary structure, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education,|
|Subjects:||Q Science > Q Science (General)
S Agriculture > SH Aquaculture. Fisheries. Angling
|Divisions:||Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology|
|Depositing User:||Karen Kornalius|
|Date Deposited:||23 Jun 2016 01:25|
|Last Modified:||23 Jun 2016 01:26|
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