Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant

Chandrasuriya, Lingam (2011) Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant. [Project Report]

[img]
Preview
PDF
Characterization of anaerobic ammonium oxidation (Anammox) bacteria (24 pages).pdf

Download (9MB) | Preview
[img] PDF (Please get the password from Digital Collection Development Unit, ext : 3932 / 3914)
Characterization of anaerobic ammonium oxidation (Anammox) bacteria (fulltext).pdf

Download (18MB)

Abstract

Anaerobic ammonium oxidation (anammox) process plays an important role in biological nitrogen removal. The bacteria catalyzing anammox process belongs to phylum Planctomycetales. The objective of this research is to characterize the anammox bacteria from leachate sample collected from a local wastewater treatment plant. Characterization of anammox bacteria includes enrichment, morphology, physico-chemical and molecular analysis. Screening for anammox bacteria was conducted using both enrichment culture and directly from leachate sample. Identification of anammox bacteria from enrichment culture and leachate sample using molecular method involves DNA extraction and PCR. Presence of anammox activity in enrichment culture was detected using physico-chemical method. No bands were obtained in peR using DNA extracted from enrichment culture. Presence of uncultured Planctomycete bacteria with potential anammox activity was detected through molecular analysis directly in leachate sample. Identification of anammox bacteria are advantageous as it can be applied in wastewater treatment plants to the level of ammonium and enhance the quality ofdischarged effluent.

Item Type: Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Anammox bacteria, biological nitrogen removal, physico-chemical analysis, molecular analysis, uncultured Planctomycete bacteria, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 19 Mar 2014 07:39
Last Modified: 13 Sep 2017 01:13
URI: http://ir.unimas.my/id/eprint/1209

Actions (For repository members only: login required)

View Item View Item