Detection and antibiotic susceptibility profiles of Listeria monocytogenes in wildlife and water samples in Kubah National Park, Sarawak, Malaysia

Lesley Maurice, Bilung and Velnetti, Linang and Fazira, A.A and Kasing, Apun and Samuel, Lihan and Micky, Vincent and Awang Ahmad Sallehin, Awang Husaini (2016) Detection and antibiotic susceptibility profiles of Listeria monocytogenes in wildlife and water samples in Kubah National Park, Sarawak, Malaysia. International Food Research Journal, 23 (1). pp. 360-365. ISSN 1985-4668

Full text not available from this repository.

Abstract

This study was conducted to detect the presence of Listeria monocytogenes (L. monocytogenes) and screen for its antibiotic susceptibility characteristic from wildlife and water samples at Kubah National Park, Sarawak, Malaysia. Samples collected were incubated and streaked on selective medium PALCAM agar to confirm the presence of Listeria spp. before they were further tested using molecular analysis. Specific Polymerase Chain Reaction (PCR) assay were performed to target specific virulence gene, haemolysin gene, hlyA to further distinguish the presence of this pathogenic bacteria in the samples. Overall, out of the 30 samples tested, 10 samples were confirmed as to contain L. monocytogenes strains and selected to subsequent antibiotic susceptibility test. Susceptibility patterns to 10 antibiotics were investigated among the L. monocytogenes strains. All strains were uniformly resistant to tetracycline and erythromycin. On the other hand, all strains were sensitive to gentamycin and tobramycin. The multiple antibiotic resistance shown by the strains in this study indicate the potential health hazard associated with the possible transmission between wildlife and water to its surrounding environment especially visitors and workers of Kubah National Park, Sarawak, Malaysia.

Item Type: Article
Uncontrolled Keywords: Antibiotic susceptibility test, HlyA gene, L. monocytogenes, Specific PCR, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 19 Apr 2016 02:13
Last Modified: 21 Oct 2016 02:32
URI: http://ir.unimas.my/id/eprint/11499

Actions (For repository members only: login required)

View Item View Item