Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus

Henry Sum, Magdline Sia and Andrew, Anna (2015) Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus. Bio Technology - An Indian Journal, 11 (3). pp. 109-114. ISSN 0974-7435

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Abstract

Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum

Item Type: Article
Uncontrolled Keywords: Chikungunya virus, Glycoprotein, Recombinant antigen, Immunoblot assay, E.coli expression system, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Ramji
Date Deposited: 01 Mar 2016 02:28
Last Modified: 01 Mar 2016 02:28
URI: http://ir.unimas.my/id/eprint/10669

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