Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates

Son, Radu and Samuel, Lihan and Azhar, Idris and Oii, Wai Ling and Muthafar Husein, Al-Haddawi and Gulam, Rusul (2000) Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. The Southeast Asian journal of tropical medicine and public health, 30 (4). pp. 760-763.

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Abstract

Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole-trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined.

Item Type: Article
Uncontrolled Keywords: Genotypic, phenotypic, Burkholderia pseudomallei, Food science, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education,research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 07 Jan 2016 06:46
Last Modified: 07 Jan 2016 06:46
URI: http://ir.unimas.my/id/eprint/10101

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